TechKnowledge

Executive summary 1.       1.   An online pharmacy is an internet based vendor of prescription           drugs, and the term in encompasses both legitimate and                illegitimate pharmacies.   2.    2.    Now Days ecommerce is growing 10% annually and with this           gigantic growth coming across new Businesses.   3.      3.  Advancement of e-commerce in India significantly opened and           area in to market most of the products which can reach almost           everybody.    4.  The sale of medicines outside the regular retail pharmacy is not           new .      5. The annual Indian pharmaceutical market is measured at around          Rs 79,0...

REAL TIME PCR   Real-time polymerase chain reaction ( qPCR ) is commonly used to measure gene expression . It is more sensitive than micro arrays in detecting small changes in expression but requires more input RNA and is less adaptable to high-throughput studies.  It is best suited for studies of small subsets of genes.  Its one major shortcoming is that the sequence of the specific target gene of interest must be known (so you can design the PCR primers),  hence real-time PCR can only be used for studying  known genes .   It is also known as a  quantitative polymerase chain reaction (qPCR) , which is a laboratory technique of molecular biology based on the polymerase chain reaction . PRINCIPLE OF REAL TIME PCR(qPCR). This same principle of amplification of PCR is employed in real-time PCR. But instead of looking at bands on a gel at the end of the reaction, the process is monitored in “real-time”. The reaction is placed into a real-time PCR machine ...

Polymerase Chain Reaction (PCR) PCR is an enzymatic process in which a specific region of DNA is replicated over and over again to yield many copies of a particular sequence. This method combines the principles of complementary nucleic acid hybridization with those of nucleic acid replication applied repeatedly through numerous cycles. The most widely used target nucleic acid amplification method is the polymerase chain reaction (PCR). This method is able to amplify a single copy of a nucleic acid target, often undetectable by standard hybridization methods, and multiply to 10 7  or more copies in a relatively short period. This thus provides ample target that can be readily detected by numerous methods. APPLICATIONS OF PCR PCR can amplify a single DNA molecule from a complex mixture, largely avoiding the need to use DNA cloning to prepare that molecule. Variants of the technique can similarly amplify a specific single RNA molecule from a complex mixture. DNA sequencing has be...