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Polymerase Chain Reaction (PCR) and its Applications

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Polymerase Chain Reaction (PCR) PCR is an enzymatic process in which a specific region of DNA is replicated over and over again to yield many copies of a particular sequence. This method combines the principles of complementary nucleic acid hybridization with those of nucleic acid replication applied repeatedly through numerous cycles. The most widely used target nucleic acid amplification method is the polymerase chain reaction (PCR). This method is able to amplify a single copy of a nucleic acid target, often undetectable by standard hybridization methods, and multiply to 10 7  or more copies in a relatively short period. This thus provides ample target that can be readily detected by numerous methods. APPLICATIONS OF PCR PCR can amplify a single DNA molecule from a complex mixture, largely avoiding the need to use DNA cloning to prepare that molecule. Variants of the technique can similarly amplify a specific single RNA molecule from a complex mixture. DNA sequencing has been greatl
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Best Food for Skin || Best food to increase STAMINA || Best Food For hair growth

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1. BEST FOOD FOR SKIN . Brocoli . Water based Fruits . Nuts . Citrus fruits . Papaya . Salmon . Mackerel . fatty fish . Carrots . Apricots . Orange 2. Best food to increase STAMINA . Eggs . Green vegetables . Banana . Almonds . Brown rice . Fish . Peanut Butter .  Citrus Fruits 3. Best Food For hair growth . Eggs . Berries . Spinach . Sweet potatoes . Avocados . walnuts . Meat . Yogurt . Blueberries
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Health Related Tips

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  1. Cold Water Benefits . Drinking 2 cups of cold water on a empty stomach can boost metabolism by 30%. 2. Concentration on work. Chew some peppermint gum. the taste of peppermint has been proven to stimulates Brain activity and help Increase our Focus. 3.KIWI. World's Strongest Food is kiwi. Taking Kiwi reduces eye problems and Increase eyesights. 4. Gram Flour. Gram floue ( Besan ) With lemon water mixed removes Pimples and adds glow to the skin. 5. Pomegranate Juice Pomegranate juice helps to gain weight and increases Blood in Huamn Beings.
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Six Powerful Attacks In Cyber world .

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  Six Big Attacks 1. DDOS Attack   * A Distributed Denial of Service  A ttack  is an attempt to make an online service unavailable by overwhelming it with traffic from multiple sources. 2. Phishing. * Phishing attack  attempt to gain sensitive, confidential information such as usernames, passwords, credit card information, network credentials, and more . 3. Backdoor. * A Backdoor  is a means to access a computer system or encrypted data that bypasses the system's customary security mechanisms.  A developer may create a backdoor so that an application or operating system can be accessed for troubleshooting or other purposes.  4. Man in the middle attack. * A Man In The Middle attack  (MITM) is an attack against a cryptographic protocol. As the name implies, in this attack the attacker sits in the middle and negotiates different cryptographic parameters with the client and the server  . 5. SQL injection. * A SQL injection  attack consists of insertion or “injection” of a SQL query v
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Some HAcKing Terms ! Generally Used

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INFOSEC   *Information security, which is the practices of Preventing unauthorized access, use, Disclosure, disruption, Modification, Inspection, Recording or destruction of information . type information or data may take any form, e.g. Electronic or physical. Infosec can also be a person who practices ethical security. OPSEC *Operation security, which is a process that identifies critical information to determine if friendly actions can be observed by enemy intelligence, determine if information obtained by Adversaries could be interpreted to be useful to them, and then executes selected measures that eliminate or reduce adversary exploitation of friendly critical information. RED TEAM *The Red Team is an Independent  Group that Challenges an organization to improve its effectiveness by assuming an adversarial role or point of view. It is particularly effective In organization With Strong Culture and Fixed ways of Approaching Problems. BLUE TEAM *A Blue team is a group of individuals
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How to activate debut video capture

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FOLLOW THESE STEPS FOR USING  DEBUT VIDEO CAPTURE SOFTWARE FOR FREE:- NO NEED TO SERIAL KEY OR REGISTARTION KEY :- 1. DOWNLOAD THE DEBUT VIDEO CAPTURE SOFTWARE FROM THE OFFICAL WEBSITE.    https://www.nchsoftware.com/capture/index.html 2. THEN INSTALL IT. 3. press win + r to open RUN.   4. Type --> "regedit"    Press Enter 5. PRESS HKEY_CURRENT_USER 6. THEN GO TO THE FILE LOCATION "NCH SUITE" THEN DEBUT 7. AND CLICK ON REGISTATION KEY 8. DELETE ALL THE FILES EXCEPT DEFAULT(NO USER ). 9. THEN CLOSED IT .  10. USE DEBUT VIDEO CAPTURE SOFTWARE WITHOUT ANY Registation KEY.
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SHORTCUT KEYS FOR PCs.

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SHORTCUT Shortcut keys help provide an easier and quicker method of navigating and executing commands in the computer software. Shortcut keys are commonly accessed by using the  Alt  key (on IBM compatible computers),  Command key  (on Apple computers),  Ctrl  key, or  Shift  key in conjunction with another key. The  de facto standard  for listing a shortcut is listing the  modifier key , a  plus symbol , and another key. In other words, "Ctrl+S" is telling you to press and hold the Ctrl key, and then press the S key too. Shortcut Keys Description Alt+F File menu options in current program. Alt+E Open  Edit  options in current program. Alt+Tab Switch between open programs. F1 View help information (F1 is used by almost every Windows program to display help). F2 Rename a selected file. F5 Refresh the current program window. Ctrl+D Bookmarks the current page in most Internet browsers. Ctrl+N Create a new or blank document in some software, or open a new  tab  in most Internet b
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SURFACE WEB, DEEP WEB, DARK WEB

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SURFACE WEB The Surface Web is the portion of the World Wide Web that is readily available to the general public and searchable with standard web search engines. It is the opposite of the deep web, the part of the web not indexed by a web search engine. DEEP WEB The deep web, invisible web, or hidden web are parts of the World Wide Web whose contents are not indexed by standard web search-engines. The opposite term to the deep web is the "surface web", which is accessible to anyone/everyone using the Internet. DARK WEB The  dark web  is the World Wide  Web  content that exists on darknets, overlay networks that use the  Internet  but require specific software, configurations, or authorization to access.
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